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Laboratory of Molecular Biology and Clinical Biochemistry

Laboratory of Molecular Biology and Clinical Biochemistry  has been developing a relevant basic and applied tasks in zootechny and veterinary medicine NAAS (National Academy of Agricultural Sciences), performs research projects and implements research results into production. From 2001 in the laboratory was operated Scientific Production Center (SPC) of the study prion infections. Verbytsky P.I., head of the State Department of Veterinary Medicine, was initiated founding of this center with director of the institute of animal biology NAAS V. V. Vlizlo. From its founding until 2015 head of the laboratory was doctor of veterinary science, professor, academician of NAAS Vlizlo V. V. Starting from 2015 head of laboratory is doctor of agricultural sciences, senior researcher Ostapiv D. D.

Laboratory staff

Vlizlo V.V - Head of Laboratory, Doctor of Veterinary Science, Professor, Academician of NAAS, Honored    Worker of Science of Ukraine.
Ostapiv D.D. - Principal Scientist, Doctor of Agricultural Sciences.
Petrukha I.M. - Senior Researcher, candidate of Agricultural Sciences.
SimonovM.R.  - Senior Fellow, PhD Veterinary Science.
Kuzmina N.V. - Junior Researcher, PhD Veterinary Science..
Vischur V.Y. - Junior Researcher, Candidate of Agricultural Sciences
Martin U.V. - junior researcher.
Bodnar J.V. - junior researcher.
Oleinik J.M. - junior researcher.
Akymyshyn N.M. - Senior Specialist.
Mikush N.U. – specialist, PhD student.
Chekh B.A. - PhD student.
Kuzmenko V.V. – PhD student
Smirnov O. O. – PhD Student.
Zathey V. – PhD Student

Laboratory function

Laboratory of Molecular Biology and Clinical Biochemistry develops fundamental and applied tasks of zootechny and veterinary medicine , and also performs scientific projects and implementing research results into production. In the laboratory works a Research and Production Center for the Study of prion infections. The head of which is a doctor of veterinary sciences, professor, academician NAAS Vlizlo V.V.
The laboratory is working on the harmonization of legislation of acts related to prion infections and other animals diseases. In particular, the center was involved in the preparation of presidential decrees "On urgent measures to ensure stable epizootic situation in Ukraine"; Resolution of the Verkhovna Rada "On State Program of diagnosis, prevention and control spongiform encephalopathy and other prion infections in Ukraine for 2005-2010"; CMU Resolution "On approval of the prevention and elimination of bovine spongiform encephalopathy and other prion infections in Ukraine for 2001-2010"; Instructions: "For diagnosis, prevention and control of spongiform encephalopathy in cattle"; Intruktsiya: "On the prevention and control of bluetongue (ovine catarrhal fever)."
In the laboratory of molecular biology and clinical biochemistry is unique equipment for the diagnosis of spongiform encephalopathy in cattle and other prion infections. The laboratory uses amino acid analyzer type Biotronik LC 6001 which is the only one in Western Ukraine
Employees of laboratory have been trained in Germany, Austria, Switzerland, France, Poland, Netherlands, Turkey and other countries.

Main  activities in the Laboratory of of Molecular Biology and Clinical Biochemistry

  • exploring  features of metabolism in animals in health and disease
  • researching  the etiology and pathogenesis, the development of informative diagnostic tests and effective measures of prevention and treatment of metabolic diseases highly productive cows
  • exploring the mechanisms of prion infections, the development of treatments and prevention of transmissible encephalopathies spongiform with using modern molecular biology techniques
  • testing and development nanoparticles  that have adjuvant properties and can be used to produce vaccines and therapeutic drugs of the new generation
  • studying biological aspects and uses of cell cultures for new veterinary drugs
  • scientific support of  livestock production.

Main tasks and grants

Our laboratory performs research program tasks for 2016 -2020 years:

  • Scientific research program № 28 "Study the molecular mechanisms and effects of nanomaterials on farm animals metabolism " 28.00.01.01.F, DR 0116U001397, chief Doctor of agrarian science Ostapiv Dmytro Dmytrovych.
  • Scientific research program № 35 "Study the metabolic processes in highly productive cows on health and disease, 35.00.02.01.F, DR 0116U001410, chief. Ph. D of veterinary science Marian Romanovych Simonov.
  • Scientific research program № 38"Development of methodology and theoretical foundations of prevention and treatment of prion infections via creating a new generation drugs using nanotechnology," 38.01.06.05.F, DR 0116U001417, chief Doctor of Veterinary Science Vlizlo Vasyl Vasylovych.

Publications

Our scientific articles published in leading in leading Ukrainian and Worldwide scientific journals: Journal of Agricultural Science; Ukrainian biochemical journal; Physiological magazine; Veterinary Medicine of Ukraine; Scientific Journal of Veterinary Medicine; The Animal Biology; Bulgarian Journal of Veterinary Medicine; Agricultural Science and Practice; Turkish Journal of Veterinary and Animal Sciences; Folia Veterinaria; Medicina veterinara. Moldova; Roczniki Naukowe Zootechniki; Hungarian Veterinary Journal; British Journal of Science, Education and Culture;. Bilogical systems; Science and Technology, International Journal of Pharmaceutical Sciences and Research.

Developments

The drug "Multyvitamiks" - used to correct microelements metabolism failure.
The drug "Viron" - used to correct and improve the immune status and post-vaccination immunity against Gumboro disease in chickens.
The drug "Pentosan polysulphate " (SP-54) - is designed to reduce prion content in animals brain, spleen and intestine.
The drug "Folihen" - used to stimulate the reproductive function in cows.
Developed and patented method of determining the physiological prion content by dot blot analysis.
Developed and patented method of determining polipleks oligonucleotides with cationic electrolytes.
The drug "Remivital" - complex therapeutic and preventive drug against cow ketosis.

Laboratory offers to make research and interpretation of the results:

hematopoiesis;
Mineral and vitamin metabolism;
protein metabolism;
histological examination of organs and tissues;
carbohydrate metabolism;
ELISA;
lipid metabolism;
Western Blot and Dot Blot analysis;
antioxidative system;
immunoprecipitation.

Laboratory constantly develop State standards of Ukraine on certification and Standards developing organizations on current issues of veterinary medicine.
Laboratory staff have published monographs, textbooks and handbooks.
Levchenko V. I., Kondrakhin I. T., Vlizlo V. V. Internal disease of animals: part 2 / Ed. by Levchenko V. I. - White Church: Bilotserkivska National Agrarian University, 2001. -544 p.
Levchenko V. I., Kondrakhin I. T., Vlizlo V. V. Clinical biochemistry in Veterinary. / Ed. by Levchenko V. I. and Halyas V. L. - White Church: Bilotserkivska National Agrarian University, 2002. - 400 p.
Burkat V. P., Vlizlo V. V., Kravtsiv R. Y. Handbook of reproductive biotechnology of cattle / Ed. by Shalovyla S. H. - Lviv: New Perspectives, 2004. - 150 p.
Levchenko V.I., Vlizlo V.V., Kondrakhin I.P., et al.Clinical diagnostics of internal diseases / ED. by Levchenko V.I. - White Church: Bilotserkivska National Agrarian University, 2004. - 608 p.
Handbook: Physiological and biochemical methods of research in biology, stockbreeding and veterinary medicine / Ed. by Vlizlo V. V. - Lviv: Institute of animal biology NAAS, 2004. – 399 p.
Verbitsky P. I., Dostoevsky P. P., Busol V. A., Vlizlo V. V., et al. Handbook for veterinarian / Ed. by Verbitsky P. I., Dostoyevsky P. P. - Kiev: The harvest, 2004. - 1280 p.
Barabash V. I., Blizno V. M., Borisenko V. V., Vlizlo V. V., et. al. Biological patterns of growth and development of farm animals / Ed. by Kozerya V. S. - Dnipropetrovsk, 2004. - 540 p.
Vlizlo V. V., Maksimovich, Halyas V. L., Leno M. I. Handbook: laboratory diagnostics in veterinary medicine. - Lviv, 2008. - 92 p.
Sologoub L. I., Antonyak H. L., Bogdanov H. A., Vlizlo V. V., Janovich V. H. Methane and the greenhouse effect of the atmosphere (environmental, biochemical and microbiological aspects). - Lviv: PАIC, 2008. - 276 p.
Vlizlo V. V., Fedoruk R. S., Ratych I. B., Iskra R. J., Gnativ V. I. History of the Institute of animal biology NAAS of Ukraine (1960 - 2010). / Ed by Vlizlo V. V., Fedoruk R. S. -   Lviv, Drohobych: Prosvit, 2010. - 324 p.
Science in the West Ukraine (1990 - 2010): Scientific publications. stockbreeding and veterinary medicine / Vlizlo V. V., Fedoruk R. S. - Lviv: PAIC, 2011. - p. 354 - 361.
Melnychuk D. O., Gryshchenko V. A., Tsvilihovskyy M. I.,  Levchenko  V. I., Golovakha V. I., Sahnyuk V. V., Kondrakhin I. P., Vlizlo V. V. et al. Veterinary clinical biochemistry program for bachelor students in speciality "Veterinary Medicine" with certification number 6.110101 in universities which have II-IV accreditation levels. - Kyiv: Agricultural education, 2011. - 19 p.
Levchenko V. I., Kondrashin I. P., Vlizlo V. V., Golovakha V. I., Sudakov M. O.,  Melnykh Y.L.,  ChumachenkoV. Y., Bohatko L. M.,  Lysenko V. V., Papchenko I. V. Animal internal diseases Part I. / Ed. by Levchenko V. I. - White Church: Bilotserkivska National Agrarian University, 2012. - 528 p.
Vlizlo V. V., Fedoruk R. S., Ratych I.B. Handbook: Laboratory methods of research in biology, stockbreeding and veterinary medicine / Ed. by Vlizlo V. V. - Lviv: SPOLOM, 2012. - 764 p.
Antonyak H. L., Vlizlo V. V., Biochemical and geochemical role of iodine: monograph. - Lviv, Ivan Franko Lviv National University, 2013. - 392 p.
Stegniy B.T., Herilovych A. P., Ibitulin I. I., Bisyuk I. J., Komisarenko S. V., Vlizlo V. V. Problems of biosafety and biological protection in veterinary medicine and biotechnology. / Ed by. Stegniy B.T. - Kharkiv: HTMT, 2013. - 414 p.
Stegniy B. T.,  Herilovych A. P., Zavgorodniy A. I., Vlizlo V. V., et. al. Molecular and genetical methods in veterinary medicine and biotechnology / Ed by. Stegniy B. T., Herilovycha A. P. - Kyiv: ST-Druk, 2014. - 286 p.
Iskra R. J., Vlizlo V. V., Fedoruk R. S., Antonyak H. L. Chromium in animals alimentation. - Kyiv: Agricultural Science, 2014. - 310 p.
Laboratory staff have published guidelines, instructions and technical conditions.
Vlizlo V. V., Levchenko V. V. Diagnostics, treatment and prevention of liver disease in cattle: guidelines. - Kyiv, 1998. - 22 p.
Verbitsky P. I.,  Pavlenko M. S.,  Oleinik L.V.,  Vlizlo V. V.,  Yarchuk B. M. spongiform encephalopathy in cattle: Guidelines. - Kyiv, 1999. - 27 p.
Vlizlo V. V., Gorbatyuk B. A.,  Coliada N. V., et al. Clinical research of animals: Guidelines for student work mplementation on clinical diagnostics. - Lviv, 2002. - 22 p.
Vlizlo V. V., Maksimovic I. A. Diagnostics, treatment and prevention of liver disease in goats: Guidelines. - Kyiv, 2003. - 27 p.
Vlizlo V. V., Maksimovic Z. A.,  Dankovych R. S., Stronsky Y. S. Biopsy application  in veterinary medicine: Guidelines. - Kyiv, 2006. - 40 p.
Vlizlo V. V., Stoyanovskyy V. H., Slivinska L. H., Maksimovic I. A.  Researching of digestive system in small animals: Guidelines. - Lviv, 2006. - p. 51.
Vlizlo V. V., Simonov M. R., Nakonechna A. M.  Immunity correction against Gumboro disease and antioxidant status in chickens cross ISA BROWN: Guidelines. - Lviv, 2007. - 24 p.
Vlizlo V. V., Gursky R. J. Сattle microelementosis in Ivano-Frankivsk region biogeochemical provinces: Guidelines - Ivano-Frankivsk, 2007. - 15 p.
Levchenko V. I., Kondrakhin I. P., Sahnyuk V. V., Horzheyev V. M., Vlizlo V. V. et. al. Internal deseases in high productive cows, etiology, diagnostics, treatment and prevention: Guidelines. - Cherkasy, 2007. - 64 p.
Vlizlo V. V.,  Verbitsky P. I., Verzhyhovskyy O. M., et. al. Diagnostics of spongiform encephalopathy in cattle: Guidelines. - Kyiv, 2007. - 61 p.
Vlizlo V.V., Rubleno N. V.,  Tyhonyuk L. A., Chornozub M. P. Аbomasum replacement  in cattle: Guidelines.  - Kyiv, 2010. - 36 p.
Vlizlo V. V., Verbitsky P. I., Moroz D. A., Lozhkina O.V., Ostapiv D.D., Petrukha I. N.,  Martin Y. V., Izyumov L. A. Prevention measures  of spongiform encephalopathy in cattle: Guidelines. - Kyiv, 2010. - 27 p.
Levchenko V. I., Slovinska L. G., Vlizlo V. V., et al. Cows anemia in west Ukraine biogeochemical zone (etiology, diagnostics, treatment and prevention): Guidelines.   - Cherkasy, 2012. - 44 p.
SuprovychT. M., Vlizlo V. V. Prediction and diagnostics of mastitis on early postnatal ontogenesis: Guidelines. - Kamenec-Podolsky: PDATU, 2013. - 38 p.
Simonov M. R.,  Vlizlo V. V.,  Petrukha I. M. Ketosis in dairy cows: Guidelines. - Lviv, 2014. - 36 p.
Verbytskyy P.I., Horzheyev V. M., Kucheryavenko O.O., Vlizlo V. V. Guidelines in diagnostics, prevention and control of spongiform encephalopathy in cattle. - Kyiv, 2002. - 7 p.
Technical conditions UA 244.30995014.001-2003. Immunomodulating drug "Antoksan" ./  Kvachov V. G.,, TA Sokyrko, Karas O. J.,  Vlizlo V. V.,  Vischur A. I.,  Ratych I. B.,  Kosenko Y. M.,  Fuks P. P. - 04.15.03. - 16 p.
Technical conditions UA 244.30995014.002-2003. The anti-inflammatory drug "Lipogen" for treating animals  ./  Vlizlo V. V.,  Kychun I. V.,  Vischur A. I.,  Andreychuk P. E.,  Yasnyska R. S. - 07.17.03. - 30 p.
Technical conditions UA 21.2-30995014-001: 2014. "Remivital." Solution for injection. Specifications. - Approved in 17.04.2014. – Lviv: State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, 2014. - 36 p.
Technical conditions UA 21.2-3099014-009: 2014. "Nanoferotsyt." Solution for injection. Specifications. - Approved in 10.06.2014. - Lviv: State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, 2014. - 15 p.
Vlizlo V.V., et al. Guidelines for prevention and control of bluetonge - Kyiv, 2009. - 6 p.
National Standard of Ukraine: Prion infection. Methods of sampling and preparation of samples for laboratory diagnosis of brain / Vlizlo V.,  Verbitsky P.,  Kulik M. et al. // DSTU 7070: 2009. Kyiv: State Committee of Ukraine for Technical Regulation and Consumer Policy, 2010. - 10 p.
Levchenko V.I., Vlizlo V.V., Maksimovic I.A.,  Abramov A.V.,  Novozhytsko Y.M. Сlinical key indicators of blood parameters and health in animals. Poster. - 2006.
Verbitsky P. I., Abramov A. V., Vlizlo V. V., Lozhkina O. V., Kalinovska I. G. Spongiform encephalopathy in cattle. Poster. - 2006.
Laboratory staff have published patents and copyright certificates.
Vlizlo V. V., Levchenko V.I. Improvement of the method of serum bilirubin definition by Iendrashika, Kleggorna and Grof. - Moscow., 1987.
Vlizlo V.V., Utechenko M.V. Improvement of the tool to puncture (biopsy) liver in cattle. - Cherkasy, 1997.
Patent for utility model: Vlizlo V. V., Ostapiv D. D. The medium for the cultivation of zygotes cows on early stages of development in vitro. Patent for utility model, UA № A61D19 / 00. – Form. № 5. - 2005.
Patent for utility model: Vlizlo V. V., Kolisnyk H. V., .Shah E. S., et al. A strain of yeast Phatfia Khodozyma In IBM-5021 - producent of carotenoids. 2006.
Patent for utility model: Vischur O. I., Vlizlo V. V., Leshovska N. M., Kuchun I.V. Drug to improve antioxidant status and immune capacity in farm animals 'Inter-flock'  - 15.12.2006. - Form. № 12. - 4 p.
Patent for invention:  Vlizlo V. V.,  Ostapiv D.D. Method of manufacturing histological oocytes and zygotes on early stages of development. Patent for invention UA 75953. C2. - Form. № 6. - 2006.
Patent for utility model: Vlizlo V. V., Simonov M. R.,  Loziuk L. V., et al. Drug with  prolonged action "Viron".  Patent for utility model UA №21297 - 2007.
Patent for utility model: Vlizlo V. V.,  Shah E. S., Kaplinskyy V. V., Boretsky N. I.,  Kolesnik M.I.,  Nechay G.I., Gural S. V. Yeast Phaffia Rhodozyma IMB Y-5026 - producent of carotenoid astaxanthin - Kyiv, 2007.
Patent for utility model: Vlizlo V. V.,  Stadnyk V. V., Major H. Y. Appliance of pentosan polysulphate (SP-54) as the inhibitory factor on the expression of physiological prion-protein complex in vitro. Patent for utility model UA 32547. - 2008.
Patent for utility model: Vlizlo V. V., Kaplinskyy V. V.,  Kuchun I. V., Yasnyskiy R. S.,  Sachko R. G.The drug to stimulate lactation in farm animals "lactogen" /  Patent for utility model UA 37709. - 2008.
Patent for utility model: Tushnytska N. Y., Grytsyniak I. I., Janovich V. G., Matvienko N. M., Pirus R. I., Vlizlo V. V. Method of treating rubella in carp. Patent for utility model UA 45510. - 2009.
Vlizlo V. V., Kostenko V. I., Ilyinskiy A. V., Luchka I. V., Salyga Y. T. Recommendations for evaluation methanogenesis and methane emissions in cattle. - Kyiv, 2011. - 34 p.
The patent for utility model: Vlizlo V. V., Stadnyk V. V., Major H. Y. Method of determining the content of physiological prion by dot blot analysis. The patent for utility model UA 56820. - 2011.
Patent for utility model: Vlizlo V. V., Kozak M. R., Ivanytska L. A. Method of detection of poliplex oligodeoxynucleotides with cationic oligoelectrolytes. Patent for utility model UA №70080. - 2012.
Patent for utility model: Vlizlo V. V., Iskra R. J. The method of correction of carbohydrate metabolism and antioxidant defense in animals. Patent for utility model UA 75010. - 2012.
Patent for utility model: Vlizlo V. V., Yasnyska R. S., Kuchun I. V., Prustupa A. I. Therapeutic drug "Hepalen". Patent for utility model UA 73865. -  2012.
Patent for utility model: Salyga Y. T., Vlizlo V. V. Method of determining the neurotoxicity of chlorpyrifos in a culture of nerve cells. Patent for utility model UA 85700. - 2013.
Patent for utility model: Simonov M. R., Vlizlo V. V., Petrukh I. M. Complex drug "Remivital". Patent for utility model UA 07582. - 2014.
Patent for utility model: Yuskiv L. L., Vlizlo V. V. Correction Method of D-vitamin status of cows in the prenatal and postnatal periods and their calves. Patent for utility model UA 08230. - 2014.
Patent for utility model: Vlizlo V. V., Iskra R. J.,  Maksimovich I. J., Berezovskiy R. Z. A method of preventing ferum-deficiency anemia in piglets. Patent for utility model UA 92917. - 2014.